Regulation of ICAM-1 in Cells of the Monocyte/Macrophage System in Microgravity
Table 1
Regulation of ICAM-1 in cells of the monocyte/macrophage system in microgravity. Overview about cell types, microgravity platforms, the experiment durations, analysis methods, number of experiments, and effects on ICAM-1.
Experimental platform
Cell types
Experimental groups
Analysis method
Number of replicates
Time of μg exposal
Regulation of ICAM-1
μg/1 g
1 g/incubator control
Clinorotation
BV2 microglia cells
μg group 1 g control group
FACS (20.000 events/sample)
= 3
24 h
Primary macrophages
μg group 1 g control group
FACS (5.000 events/sample)
= 3
1–5 d
U937 macrophages
μg group 1 g control group
FACS (10.000 events/sample)
= 6
1–5 d
Parabolic flight
BV2 microglia cells
μg group 1 g control group
Confocal microscopy/ random sampling module (>500 cells/sample)
= 3 (w/o)
= 3 (with PMA)
20 sec
= 3 (with TNF-a)
—
U937 macrophages
μg group 1 g control group Ground control
FACS (10.000 events/sample)
= 9
20 sec
= 9
= 6
U937 monocytes
μg group 1 g control group Ground control
FACS (10.000 events/sample)
= 9 (w/o PMA)
20 sec
—
—
= 5 (with PMA)
= 12 (w/o PMA)
= 6 (with PMA)
= 2 (w/o PMA)
= 1 (with PMA)
μg group 1 g control group 1.8 g control group Hardware control