Neonatal Death and Heart Failure in Mouse with Transgenic HSP60 Expression

Figure 7

TUNEL staining and activated caspase-3 detection in neonatal heart sections. (a) Detection of nuclear DNA fragmentation with TUNEL reaction in the left ventricle sections of neonatal H⁺/C⁺ and H⁺/C⁻ mice. Fluorescein-12-dUTP (TdT) and DAPI were used for TUNEL and nuclear staining. (b) Immunofluorescence detection of activated (cleaved) caspase-3 induction in H⁺/C⁺ and in H⁺/C⁻ heart sections. Scale bar = 50 μm.

(a)

(b)