Acoustic-Frequency Vibratory Stimulation Regulates the Balance between Osteogenesis and Adipogenesis of Human Bone Marrow-Derived Mesenchymal Stem Cells

Figure 4

Effects of acoustic-frequency vibratory stimulation (AFVS) on lipid accumulation in adipocyte-like cells. Human bone marrow-derived mesenchymal stem cells (BM-MSCs) were incubated in adipogenic differentiation medium for 21 days and stimulated with AFVS at the frequencies of 30 Hz, 400 Hz, and 800 Hz. Cells in static culture condition (0 Hz) served as a control. (a) At the time point of day 21, lipid accumulation was assessed by Oil Red O staining. Scale bar = 200 μm. The lipid-bound stain at the time point of day 14 (b) and 21 (c), respectively, was dissolved in isopropanol and quantified via a spectrophotometer. Values are mean ± standard error of four independent experiments (). , in the indicated groups from unpaired -test. , compared with the 0 Hz control group from unpaired -test.

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