Acoustic-Frequency Vibratory Stimulation Regulates the Balance between Osteogenesis and Adipogenesis of Human Bone Marrow-Derived Mesenchymal Stem Cells

Table 1

Primers used for real-time RT-PCR.

Gene	Primer sequence (5′-3′)	GeneBank accession
The internal standard gene
GAPDH	F: AGAAAAACCTGCCAAATATGATGAC	NM_002046
	R: TGGGTGTCGCTGTTGAAGTC
Osteogenic marker genes
COL1A1	F: CAGCCGCTTCACCTACAGC	NM_000088.3
	R: TTTTGTATTCAATCACTGTCTTGCC
ALP	F: AGCACTCCCACTTCATCTGGAA	NM_000478.3
	R: GAGACCCAATAGGTAGTCCACATTG
SPP1/OPN	F: GCGAGGAGTTGAATGGTG	NM_013227.2
	R: CTTGTGGCTGTGGGTTTC
RUNX2	F: AGAAGGCACAGACAGAAGCTTGA	NM_001015051.3
	R: AGGAATGCGCCCTAAATCACT
Adipogenic marker genes
FABP4	F: AACCTTAGATGGGGGTGTCCTG	NM_001442.2
	R: TCGTGGAAGTGACGCCTTTC
CEBPA	F: CGGTGGACAAGAACAGCAAC	NM_004364
	R: CGGAATCTCCTAGTCCTGGC
LEP/Leptin	F: ATGACACCAAAACCCTCATCAA	NM_000230.2
	R: GAAGTCCAAACCGGTGACTTT
PPARG2	F: CCTATTGACCCAGAAAGCGATT	NM_015869.4
	R: CATTACGGAGAGATCCACGGA