Current Advances in the Application of Raman Spectroscopy for Molecular Diagnosis of Cervical Cancer

Figure 1

Schematic showing the process involved in Raman spectra collection. When the sample is illuminated by an incident monochromatic light, the majority of the scattered light is of the same wavelength—elastically scattered (green arrow). A notch filter is therefore used to block the elastically scattered light which would otherwise overwhelm the weak signal of the Raman or inelastically scattered light (orange arrow). The Raman scattered light may be dispersed according to wavelength through a grating and detected by a CCD (charge-coupled device) detector. A Raman spectrum is finally shown upon software analysis.