Targeted Next-Generation Sequencing Reveals Hot Spots and Doubly Heterozygous Mutations in Chinese Patients with Familial Cardiomyopathy

Table 2

PCR primers for amplification of the mutation sites.

Gene symbol	Nucleotide change	Exon	Primer (5′-3′)	Tm (°C)	Fragment size (bp)
TNNI3	c.433C>G	7	Sense: GCCTAAGCCGGGAAGAGACTGGTA	55	437
			Antisense: GAGGACCCCTTACTAGCTGCTTCT
LMNA	c.568C>T	3	Sense: GAGTAGCTGGGACTACAGGCGTGT	57	1338
			Antisense: ATCTGACTCCACATCCTGCGACC
MYH7	c.3134G>A	25	Sense: GGCAATCTCACAGTCCCCTAATAA	55	508
			Antisense: TTTTTGCCAGGGAGGACCATCTAA