Vitamin E Supplementation Delays Cellular Senescence In Vitro

Figure 4

Acute vitamin E treatment downregulates the expression of the P21 gene in HUVEC cultures at passage 9. Gene expression analysis of HUVEC and human fibroblasts cultures at different passages and following vitamin E treatment. Error bars represent s.e.m. All values were normalized to the expression of the housekeeping gene 18S. Gene expression is indicated as fold change (f.c.). (a) P16 gene expression analysis in HUVECs. Passage 3: CTR = 1.0 , vitamin E = 1.0 ; test: (ns). Passage 9: CTR = 8.5, , vitamin E = 7.7, ; test, (ns). Passage 3 CTR versus passage 9 CTR: test, . (b) P21 gene expression in HUVECs. Passage 3: CTR = 1.0 (), vitamin E = 1.1 (); test: (ns). Passage 9: CTR = 1.5 (), vitamin E = 1.1 (); test, . Passage 3 CTR versus passage 9 CTR: test, . (c) P21 gene expression in human fibroblasts. For all analyses . CTRs were already reported and described in Figure 2. Passage 10: CTR = 1.1, vitamin E = 1.0; test: (ns). Passage 33: CTR = 1.7, vitamin E = 1.7; test, (ns). (d) P16 gene expression in human fibroblasts. For all analyses . CTRs were already reported and described in Figure 2. Passage 10: CTR = 1.0, vitamin E = 1.0; test: (ns). Passage 33: CTR = 1.0, vitamin E = 1.0; test, (ns).

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