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BioMed Research International
Volume 2015, Article ID 563247, 11 pages
Research Article

Vitamin E Supplementation Delays Cellular Senescence In Vitro

DSM Nutritional Products Ltd., R & D Human Nutrition and Health, P.O. Box 2676, 4002 Basel, Switzerland

Received 31 July 2015; Accepted 8 October 2015

Academic Editor: Paul J. Higgins

Copyright © 2015 Giorgio La Fata et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1 compares the nuclei size and proliferation rate of HUVEC cultures at passage 3 and 10. Supplementary Figure 2 compares the phenotype and the behavior of human fibroblasts at early and late passages. Supplementary Figure 3 describes the gene expression analysis (for the P16 and P21 mRNAs) performed in human fibroblasts at early and late passages. Supplementary Figure 4 shows the proliferation analysis using Ki67 as a marker for proliferation. Supplementary Figure 5 shows the effect of a chronic vitamin E supplementation in human fibroblasts during the progression of cellular senescence.

  1. Supplementary Material