BioMed Research International / 2015 / Article / Tab 1

Research Article

Identification of a New Alcaligenes faecalis Strain MOR02 and Assessment of Its Toxicity and Pathogenicity to Insects

Table 1

Mortality assessment in the pathogenicity assay of A. faecalis MOR02 and E. coli DH5α (carrying plasmid pT4-mCherry) on G. mellonella larvae.

Larvae treatmentMortality (%)
A. faecalis MOR02-Cherry24 h after injection48 h after injection72 h after injection>96 h after injection

Puncture0000
LB broth0000
240 CFU003.33 ± 4.710
2,400 CFU06.67 ± 4.71a6.67 ± 9.43a33.33 ± 9.42a
24,000 CFU96.67 ± 4.71a3.33 ± 4.71a**
240,000 CFU100a***

E. coli DH5α24 h after injection48 h after injection72 h after injection>96 h after injection

Puncture01000
LB broth0000
240 CFU0000
2,400 CFU0000
24,000 CFU0000
240,000 CFU0000

Significant differences between values. Note: the percentage values shown are accumulative. *At this time all larvae were dead.