Helicobacter pylori Outer Membrane Protein 18 (Hp1125) Is Involved in Persistent Colonization by Evading Interferon-γ Signaling
Δomp18 H. pylori induces more nitric oxide (NO) production in macrophages and mice stomachs. The murine macrophage RAW264.7 was used in this experiment and was infected by H. pylori 26695 or Δomp18 H. pylori 26695 with or without IFN-γ (50 pg/mL). (a) Western blot analysis of phosphorylated signal transducer and regulator of transcription 1 (STAT1) protein expression in murine macrophage RAW264.7 cells. Actin was a loading control. (b) Analysis of NO secretion from murine macrophage RAW264.7 cells by ELISA. (c) Analysis of NO secretion in gastric tissues from C57BL/6 mice infected by H. pylori SS1 and Δomp18 H. pylori SS1 at week 8 by ELISA. /group, 6–8 weeks old. . Data are means ± SEM from 3 independent experiments.