Preparation, Optimization, and Screening of the Effect of Processing Variables on Agar Nanospheres Loaded with Bupropion HCl by a D-Optimal Design

Table 2

Regression analysis for particle size, zeta potential, drug loading efficiency, and RE₃₀₀%.

Responses
Factor	Particle size			Zeta potential			Drug loading efficiency %			RE300%
	C.E	Std. error	value	C.E	Std. error	value	C.E	Std. error	value	C.E	Std. error	value
Int	+561.70	24.58	0.1004	−29.37	0.64	0.0118	+33.70	1.03	<0.0001	+81.31	3.06	0.0289
	—	—	—	+1.27	0.73	0.0969	—	—	—	+0.84	1.48	0.5760
	−60.02	30.53	0.0626	—	—	—	—	—	—	+0.73	1.59	0.6516
	−3.45	27.94	0.9030	—	—	—	+6.31	1.18	<0.0001	−0.28	1.43	0.8456
	—	—	—	+0.34	0.72	0.6386	−3.21	1.15	0.0106	—	—	—
	—	—	—	—	—	—	—	—	—	5.29	3.70	0.1708
	—	—	—	—	—	—	—	—	—	5.88	2.88	0.0565
	—	—	—	—	—	—	—	—	—	−11.87	3.70	0.0052
	—	—	—	—	—	—	—	—	—	3.87	1.52	0.0211
	—	—	—	−2.41	0.78	0.0053	—	—	—	—	—	—
	+66.11	32.73	0.0563	—	—	—	—	—	—	—	—	—
Lack of fit values
value	0.52			1.05			2.78			1.18
value	0.8524			0.5270			0.1315			0.4595

: CaCl₂ percent, : homogenization speed, : agar percent, : HPCD percent, C.E: coefficient estimate in terms of actual factors, and Int: intercept; the positive sign of the factor represents a synergistic effect on the response, while a negative sign means an antagonist relationship.