Mcl-1 Is a Novel Target of miR-26b That Is Associated with the Apoptosis Induced by TRAIL in HCC Cells

Figure 4

Mcl-1 abolished the sensitization of miR-26b to TRAIL-induced cytotoxicity. Three independent experiments were performed. (a) HepG2 cells were transfected with indicated RNA oligos with/without TRAIL. Then the MTT assay was performed for evaluating the cell viability. versus NC oligo group. (b) HepG2 cells were transfected with indicated RNA oligos with/without TRAIL. Then the trypan blue exclusion assay was performed for evaluating the cell death. versus NC oligo group. (c) HepG2 cells were transfected with indicated RNA oligos with/without TRAIL. Then the cell apoptosis was measured using Annexin V/PI staining on flow cytometry.

(a)

(b)

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