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Target organism | Forward primer | Reverse primer | TaqMan probe |
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Multiplex-PCR targeting invasive enteric bacteria |
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Salmonella spp. | 5′-ATT-GTT-GAT-TCA-GGT-ACA-AAC-3′ | 5′-AAT-TAG-CCA-TGT-TGT-AAT-CTC-3′ | 5′-CAA-GTT-CAA-CGC-GCA-ATT-TA-3′ |
Shigella spp./EIEC | 5′-CAG-AAG-AGC-AGA-AGT-ATG-AG-3′ | 5′-CAG-TAC-CTC-GTC-AGT-CAG-3′ | 5′-ACA-GGT-GAT-GCG-TGA-GAC-TG-3′ |
Campylobacter jejuni | 5′-CTA-TAA-CAA-CTG-CAC-CTA-CTA-AT-3′ | 5′-AAG-TGT-AAG-CAC-ACA-AGG-TA-3′ | 5′-CTT-AAT-AGC-CGT-CAC-CCC-AC-3′ |
Yersinia spp. | 5′-GCA-TTA-ACG-AAT-ATG-TTA-GC-3′ | 5′-ATC-GAG-TTT-GGA-GTA-TTC-AT-3′ | 5′-CCG-CTT-CCA-AAT-TTT-GTC-AT-3′ |
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Multiplex-PCR targeting enteric protozoa |
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Entamoeba histolytica | 5′-ATT-GTC-GTG-GCA-TCC-TAA-CTC-A-3′ | 5′-GCG-GAC-GGC-TCA-TTA-TAA-CA-3′ | 5′-TCA-TTG-AAT-GAA-TTG-GCC-ATT-T-3′ |
Giardia duodenalis | 5′-GAC-GGC-TCA-GGA-CAA-CGG-TT-3′ | 5′-TTG-CCA-GCG-GTG-TCC-G-3′ | 5′-CCC-GCG-GCG-GTC-CCT-GCT-AG-3′ |
Cyclospora cayetanensis | 5′-TAG-TAA-CCG-AAC-GGA-TCG-CAT-T-3′ | 5′-AAT-GCC-ACG-GTA-GGC-CAA-TA-3′ | 5′-CCG-GCG-ATA-GAT-CAT-TCA-AGT-TTC-TGA-CC-3′ |
Cryptosporidium parvum | 5′-CGC-TTC-TCT-AGC-CTT-TCA-TGA-3′ | 5′-CTT-CAC-GTG-TGT-TTG-CCA-AT-3′ | 5′-CCA-ATC-ACA-GAA-TCA-TCA-GAA-TCG-ACT-GGT-ATC-3′ |
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Internal control PCR targeting phocid herpesvirus DNA |
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Phocid herpes virus | 5′-GGG-CGA-ATC-ACA-GAT-TGA-ATC-3′ | 5′-GCG-GTT-CCA-AAC-GTA-CCA-A-3′ | 5′-TTT-TTA-TGT-GTC-CGC-CAC-CAT-CTG-GATC-3′ |
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