BioMed Research International / 2015 / Article / Tab 1

Research Article

Surveillance of Food- and Smear-Transmitted Pathogens in European Soldiers with Diarrhea on Deployment in the Tropics: Experience from the European Union Training Mission (EUTM) Mali

Table 1

Sequences of the primer-probe-sets used in the applied real-time PCR assays [59].

Target organismForward primerReverse primerTaqMan probe

Multiplex-PCR targeting invasive enteric bacteria

Salmonella spp.5′-ATT-GTT-GAT-TCA-GGT-ACA-AAC-3′5′-AAT-TAG-CCA-TGT-TGT-AAT-CTC-3′5′-CAA-GTT-CAA-CGC-GCA-ATT-TA-3′
Shigella spp./EIEC5′-CAG-AAG-AGC-AGA-AGT-ATG-AG-3′5′-CAG-TAC-CTC-GTC-AGT-CAG-3′5′-ACA-GGT-GAT-GCG-TGA-GAC-TG-3′
Campylobacter jejuni 5′-CTA-TAA-CAA-CTG-CAC-CTA-CTA-AT-3′5′-AAG-TGT-AAG-CAC-ACA-AGG-TA-3′5′-CTT-AAT-AGC-CGT-CAC-CCC-AC-3′
Yersinia spp.5′-GCA-TTA-ACG-AAT-ATG-TTA-GC-3′5′-ATC-GAG-TTT-GGA-GTA-TTC-AT-3′5′-CCG-CTT-CCA-AAT-TTT-GTC-AT-3′

Multiplex-PCR targeting enteric protozoa

Entamoeba histolytica 5′-ATT-GTC-GTG-GCA-TCC-TAA-CTC-A-3′5′-GCG-GAC-GGC-TCA-TTA-TAA-CA-3′5′-TCA-TTG-AAT-GAA-TTG-GCC-ATT-T-3′
Giardia duodenalis 5′-GAC-GGC-TCA-GGA-CAA-CGG-TT-3′5′-TTG-CCA-GCG-GTG-TCC-G-3′5′-CCC-GCG-GCG-GTC-CCT-GCT-AG-3′
Cyclospora cayetanensis 5′-TAG-TAA-CCG-AAC-GGA-TCG-CAT-T-3′5′-AAT-GCC-ACG-GTA-GGC-CAA-TA-3′5′-CCG-GCG-ATA-GAT-CAT-TCA-AGT-TTC-TGA-CC-3′
Cryptosporidium parvum 5′-CGC-TTC-TCT-AGC-CTT-TCA-TGA-3′5′-CTT-CAC-GTG-TGT-TTG-CCA-AT-3′5′-CCA-ATC-ACA-GAA-TCA-TCA-GAA-TCG-ACT-GGT-ATC-3′

Internal control PCR targeting phocid herpesvirus DNA

Phocid herpes virus5′-GGG-CGA-ATC-ACA-GAT-TGA-ATC-3′5′-GCG-GTT-CCA-AAC-GTA-CCA-A-3′5′-TTT-TTA-TGT-GTC-CGC-CAC-CAT-CTG-GATC-3′