The Effects of TiO₂ Nanodot Films with RGD Immobilization on Light-Induced Cell Sheet Technology

Figure 5

(a) Cell apoptosis and death of samples were tested by flow cytometry after culturing for 7 days. (b) Cell number of normal, apoptosis, or death cells has been counted. There is statistic difference when TN with UV group compared to any of the other three groups. . (c) Viability of MC3T3-E1 cell sheets cultured on TN samples or TR samples staining by Hoechst-PI double-labeled method after UV365 for 30 min. MC3T3-E1 cell sheets cultured on PS samples after 65°C water bath were stained as control group. Scale bar: 100 μm. (d) Attachment and proliferation ability of reseeded MC3T3-E1 cells were tested by cck-8. The abscissa indicated the cultured days for cells. .

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