Community Structure of Ammonia-Oxidizing Archaea and Ammonia-Oxidizing Bacteria in Soil Treated with the Insecticide Imidacloprid

Table 2

Primers used for molecular analyses in this study.

Targets	Primers	Sequence (5′-3′)	Length of amplicon (bp)	References
AOA	Arch-amoAF	STA ATG GTC TGG CTT AGA CG	635	[34]
	arch-amoAR	GCG GCC ATC CAT CTG TAT GT
AOB	amoA-1F	GGG GTT TCT ACT GGT GGT	491	[35]
	amoA-2R	CCC CTC KGS AAA GCC TTC TTC

AOA: ammonia-oxidizing archaea; AOB: ammonia-oxidizing bacteria. Forty-base pair GC-clamp, CGC CCG CCG CGC GCG GCG GGC GGG GCG GGG GCA CGG GGG C [36], was attached to the 5′ end of the forward primers.