Research Article
Community Structure of Ammonia-Oxidizing Archaea and Ammonia-Oxidizing Bacteria in Soil Treated with the Insecticide Imidacloprid
Table 3
The two-way ANOVA analysis for the measured parameters.
| Parameter | Source of variation | df | Sum of squares | Mean squares | Variance explained (%) | | |
| Concentration of ions | | | | | | | | | Treatment | 2 | 1908.79 | 954.40 | 83 | 989.3 | < 0.001 | Time | 3 | 158.20 | 52.73 | 7 | 54.6 | < 0.001 | Treatment × time | 6 | 204.12 | 34.02 | 9 | 35.3 | < 0.001 | | Treatment | 2 | 819.2 | 409.6 | 64 | 2275.5 | < 0.001 | Time | 3 | 282.2 | 94.1 | 22 | 522.6 | < 0.001 | Treatment × time | 6 | 13.9 | 28.9 | 13 | 161.1 | < 0.001 | Number of nitrifying bacteria | Treatment | 2 | 15.65 | 7.83 | 74 | 124.7 | < 0.001 | Time | 3 | 2.23 | 0.74 | 10 | 11.8 | < 0.001 | Treatment × time | 6 | 1.63 | 0.27 | 8 | 4.3 | = 0.004 | AOA-DGGE analysis | | | | | | | | | Treatment | 2 | 0.21 | 0.11 | 64 | 61.2 | < 0.001 | Time | 3 | 0.02 | 0.01 | 8 | 4.8 | = 0.009 | Treatment × time | 6 | 0.05 | 0.001 | 16 | 5.1 | = 0.002 | | Treatment | 2 | 24.50 | 12.25 | 72 | 73.5 | < 0.001 | Time | 3 | 0.66 | 0.22 | 2 | 1.3 | = 0.286 | Treatment × time | 6 | 4.83 | 0.81 | 14 | 4.8 | = 0.002 | | Treatment | 2 | 0.002 | 0.0009 | 24 | 7.1 | = 0.004 | Time | 3 | 0.001 | 0.0004 | 15 | 3.0 | = 0.052 | Treatment × time | 6 | 0.002 | 0.0003 | 20 | 2.0 | = 0.112 | AOB-DGGE analysis | | | | | | | | | Treatment | 2 | 0.52 | 0.26 | 34 | 214.6 | < 0.001 | Time | 3 | 0.46 | 0.15 | 30 | 126.3 | < 0.001 | Treatment × time | 6 | 0.53 | 0.09 | 35 | 72.9 | < 0.001 | | Treatment | 2 | 7.72 | 3.86 | 28 | 139.0 | < 0.001 | Time | 3 | 9.42 | 3.14 | 34 | 113.0 | < 0.001 | Treatment × time | 6 | 9.83 | 1.63 | 36 | 59.0 | < 0.001 | | Treatment | 2 | 0.0012 | 0.0005 | 10 | 2.5 | = 0.102 | Time | 3 | 0.0009 | 0.0003 | 8 | 1.4 | = 0.267 | Treatment × time | 6 | 0.0039 | 0.0007 | 36 | 3.1 | = 0.023 |
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AOA: ammonia-oxidizing archaea; AOB: ammonia-oxidizing bacteria; : Shannon-Wiener index; : richness; : evenness. The effects in bold are significant at < 0.05.
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