Decreased Pulmonary Function in School Children in Western Japan after Exposures to Asian Desert Dusts and Its Association with Interleukin-8

Figure 4

(a) IL-8 transcriptional activity in THP-G8 cells stimulated with LPS at various concentrations () for 5 h. IL-8 transcriptional activity is based on normalized SLO luciferase activity (nSLO-LA), which was calculated as SLO-LA divided by SLR-LA. The fold induction of nSLO-LA was calculated as the nSLO-LA of treated cells divided by that of untreated cells [18]. (b) IL-8 transcriptional activity in THP-G8 cells stimulated with 100 ng/mL LPS () for various time periods. (c) Concentrations of IL-8 in supernatants of a stable THP-1-derived IL-8 reporter cell line stimulated with solvent only (negative control), LPS (, 1 ng/mL), and LPS (, 100 ng/mL). The IL-8 concentration was measured using an ELISA kit. Samples were run in triplicate. The assay range was 31.2 to 2000 pg/mL.

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