BioMed Research International / 2015 / Article / Fig 4

Research Article

Decreased Pulmonary Function in School Children in Western Japan after Exposures to Asian Desert Dusts and Its Association with Interleukin-8

Figure 4

(a) IL-8 transcriptional activity in THP-G8 cells stimulated with LPS at various concentrations () for 5 h. IL-8 transcriptional activity is based on normalized SLO luciferase activity (nSLO-LA), which was calculated as SLO-LA divided by SLR-LA. The fold induction of nSLO-LA was calculated as the nSLO-LA of treated cells divided by that of untreated cells [18]. (b) IL-8 transcriptional activity in THP-G8 cells stimulated with 100 ng/mL LPS () for various time periods. (c) Concentrations of IL-8 in supernatants of a stable THP-1-derived IL-8 reporter cell line stimulated with solvent only (negative control), LPS (, 1 ng/mL), and LPS (, 100 ng/mL). The IL-8 concentration was measured using an ELISA kit. Samples were run in triplicate. The assay range was 31.2 to 2000 pg/mL.
(a)
(b)
(c)

We are committed to sharing findings related to COVID-19 as quickly as possible. We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. Review articles are excluded from this waiver policy. Sign up here as a reviewer to help fast-track new submissions.