Research Article

Isolation, Culture, and Characterization of Chicken Cartilage Stem/Progenitor Cells

Figure 7

Adipogenic differentiation of chicken CSPCs. (a)–(c) After 2 weeks of induction, CSPCs metamorphosed from fibroblast-like shapes to oblate shapes and formed many lipid droplets in cells. Along with the prolongation of inducing time, droplets increased and aggregated to form larger ones gradually. (d)–(f) Lipid droplets displayedred through oil red O staining. (g) The expressions of adipocyte specific genes, including LPL and PPAR-γ, were detected by RT-PCR in the induced group after incubation for 2 weeks and these genes were all positive.
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