Isolation, Culture, and Characterization of Chicken Cartilage Stem/Progenitor Cells

Table 1

Primer sequences used in RT-PCR assay.

Gene name	Primer sequences	Product length (bp)	Tm (°C)
GADPH	F: 5′ CACAGAAGACGGTGGATG 3′	314	50
	R: 5′ ACCATTGAAGTCACAGGAG 3′
COL2A1	F: 5′ GCGGAGATTACTGGATTGA 3′	293	50
	R: 5′ GTGGTAGGTGACGTTCTG 3′
VIM	F: 5′ GCGTTGCTGCTAAGAATC 3′	141	50
	R: 5′ ACTGAATCTGTCTGCGATAT 3′
SOX 9	F: 5′ GAGGCTGCTGAATGAGAG 3′	227	50
	R: 5′ TGATGCTGGAGGATGACT 3′
ACAN	F: 5′ ATGCCGTTATTGCCACTC 3′	110	60
	R: 5′ TACACATCGTAGGTCTCTGA 3′
PPAR-γ	F: 5′ CTGTCTGCGATGGATGAT 3′	199	47.3
	R: 5′ AATAGGGAGGAGAAGGAG 3′
LPL	F: 5′ AGTGAAGTCAGGCGAAAC 3′	477	48.7
	R: 5′ ACAAGGCACCACGATT 3′
COL1A2	F: 5′ AATGGTGGCGGATATGAAG 3′	190	50
	R: 5′ GGTGGCTAAGTCTGAGGT 3′
SSPP1	F: 5′ CACTTCAGCAGACTCATTAC 3′	411	50
	R: 5′ TCTCTACGCTCTGATGTTG 3′