The Rapid Inactivation of Porcine Skin by Applying High Hydrostatic Pressure without Damaging the Extracellular Matrix

Figure 1

Quantification of the mitochondrial activity of the pressurized skin measured by the WST-8 assay. The horizontal axis shows the pressure, solution, and pressurization time in order. NSS shows the absorbance of the skin that was preserved in the NSS without pressurization. 100_NSS5 means the skin immersed in NSS pressurized at 100 MPa for 5 minutes and 100_DW5 means the skin immersed in DW pressurized at 100 MPa for 5 minutes. A significant difference is shown in the figure. The absorbance was significantly decreased in the skin immersed in the DW at the pressure of 100 MPa, and in the NSS and the DW at the pressure of 200 MPa, compared with that in NSS without pressurization (). The absorbance for the skin in the NSS at the pressure of 200 MPa for 10, 30, and 60 min and in the DW at the pressure of 200 MPa was significantly decreased compared with that in NSS at 100 MPa for 10, 30, and 60 min (). The activity of the skin immersed in NSS at the pressure of 200 MPa and in DW at the pressure of 100 and 200 MPa showed no activity level.