The Rapid Inactivation of Porcine Skin by Applying High Hydrostatic Pressure without Damaging the Extracellular Matrix

Figure 2

Quantification of mitochondrial activity of punched-out skin from the pressurized skin measured by a WST-8 assay. Horizontal axis shows the pressure and solution in order. The NSS shows the absorbance of the skin preserved in the NSS without pressurization. 100_NSS means the skin sample was punched out from the skin and immersed in NSS and pressurized at 100 MPa and 0_DW means the skin was punched out from the skin and immersed in DW without pressurization. A significant difference is shown in the figure. The absorbance of 200_NSS was decreased compared with those of NSS, 100_NSS, and 0_DW (). The absorbance levels of 100_DW and 200_DW were significantly lower than those of NSS and 100_NSS (). The absorbance levels of 200_NSS and 200_DW showed no activity level.