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BioMed Research International
Volume 2015 (2015), Article ID 587465, 5 pages
http://dx.doi.org/10.1155/2015/587465
Research Article

APOE Gene Polymorphism and Risk of Coronary Stenosis in Pakistani Population

1Atta-Ur-Rahman School of Applied Biosciences, National University of Sciences & Technology, H/12, Islamabad 44000, Pakistan
2Department of Human Genetics, University of Pittsburgh, Pittsburgh, PA 15261, USA
3Department of Cardiology, Lady Reading Hospital, Peshawar 25000, Pakistan

Received 26 January 2015; Accepted 15 March 2015

Academic Editor: Sarah H. Elsea

Copyright © 2015 Asma Naseer Cheema et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Genetic variation in lipid regulatory genes, particularly APOE, significantly influences the risk of coronary artery disease (CAD). This study aimed to assess the association between APOE polymorphism and angiographically assessed coronary stenosis in Pakistani population. A total of 695 subjects (22.3% female, mean age = years) presenting with chest pain were enrolled after obtaining written informed consent. CAD stenosis/extent was assessed by angiography. Patients were classified as having severe stenosis (≥70%), moderate stenosis (30–69%), and mild stenosis (<30%). CAD patients with ≥70% stenosis () were further categorized based on possessing one, two, or three vessel diseases to assess the disease extent. Genomic DNA from leukocytes was isolated with DNA purification kit (Qiagen) and APOE polymorphisms (E2/E3/E4) were determined using TaqMan assays. Six hundred and seventy-two of 695 subjects were successfully genotyped. The frequency of APOE4 carriers (3/4 and 4/4 genotypes) was significantly higher in severe stenosis group (≥70%) as compared to mild group (<30%) (22.8% versus 13.01%; ). In multiple regression, the odds ratio for APOE4 carriers to develop ≥70% stenosis was 2.16 (95% CI: 1.29–3.79; ). In conclusion, the presence of APOE4 allele is a significant risk factor to develop severe coronary stenosis (>70%) among Pakistanis.