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BioMed Research International
Volume 2015, Article ID 589471, 11 pages
http://dx.doi.org/10.1155/2015/589471
Research Article

Blood or Urine IP-10 Cannot Discriminate between Active Tuberculosis and Respiratory Diseases Different from Tuberculosis in Children

1Translational Research Unit, Department of Epidemiology and Preclinical Research, National Institute for Infectious Diseases (INMI), 00149 Rome, Italy
2Italian Association for Solidarity among People (AISPO), P.O. Box 7146, Kampala, Uganda
3St. Francis Nsambya Hospital, P.O. Box 7146, Kampala, Uganda
4Swiss Tropical and Public Health Institute, Medical Services and Diagnostic Department, 4002 Basel, Switzerland
5University of Basel, 4003 Basel, Switzerland
6Ifakara Health Institute, Bagamoyo Research and Training Center, P.O. Box 78373, Bagamoyo, Tanzania
7Department of Epidemiology and Preclinical Research, National Institute for Infectious Diseases (INMI), 00149 Rome, Italy

Received 13 May 2015; Revised 8 July 2015; Accepted 14 July 2015

Academic Editor: Subash Babu

Copyright © 2015 Linda Petrone et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objectives. Interferon-γ inducible protein 10 (IP-10), either in blood or in urine, has been proposed as a tuberculosis (TB) biomarker for adults. This study aims to evaluate the potential of IP-10 diagnostics in children from Uganda, a high TB-endemic country. Methods. IP-10 was measured in the blood and urine concomitantly taken from children who were prospectively enrolled with suspected active TB, with or without HIV infection. Clinical/microbiological parameters and commercially available TB-immune assays (tuberculin skin test (TST) and QuantiFERON TB-Gold In-Tube (QFT-IT)) were concomitantly evaluated. Results. One hundred twenty-eight children were prospectively enrolled. The analysis was performed on 111 children: 80 (72%) of them were HIV-uninfected and 31 (27.9%) were HIV-infected. Thirty-three healthy adult donors (HAD) were included as controls. The data showed that IP-10 is detectable in the urine and blood of children with active TB, independent of HIV status and age. However, although IP-10 levels were higher in active TB children compared to HAD, the accuracy of identifying “active TB” was low and similar to the TST and QFT-IT. Conclusion. IP-10 levels are higher in children with respiratory illness compared to controls, independent of “TB status” suggesting that the evaluation of this parameter can be used as an inflammatory marker more than a TB test.