Transcriptomic and Immunohistochemical Profiling of SLC6A14 in Pancreatic Ductal Adenocarcinoma

Figure 4

Histology of CT-isoattenuating tumor. (a–c) Note the diffusively infiltrative invasive adenocarcinoma with ductal morphology (open arrows), the relatively intact lobular architecture of the residual normal pancreas (solid arrows), and the regions of fibrosis with sparse cellularity (area between arrows). Scale bar equals 1 mm. (a) KRT19 immunohistochemistry of a formalin-fixed, paraffin-embedded (FFPE) section. (b) Hematoxylin-eosin stain of a frozen section from the same patient. An adjacent section from this same frozen block was used for laser capture microdissection. (c) An adjacent section from the FFPE block shown in panel (a) stained for SLC6A14. (d) Higher magnification of region of panel (c) (rectangle) shows moderate cytoplasmic SLC6A14 staining in adenocarcinoma cells (solid arrowhead), while the adjacent normal acinar cells and a normal duct (open arrowhead) are unstained. Strong cytoplasmic/membranous staining was also observed in scattered stromal cells. Scale bar equals 200 μm.

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