Primary Murine Myotubes as a Model for Investigating Muscular Dystrophy

Figure 2

Muscle fiber transduction via lentiviruses. (a) In 72 h after muscle fibers transduction via nonconcentrated LV encoded LMNA (1 hour incubation with LV) muscle fibres kept their cross-striated pattern (upper panel). When concentrated LV encoded GFP was applied muscle fibers acquired positive signal, however, lost their cross-striation (lower panel). Satellite cells in both applications were positively transduced. (b) In 72 h after muscle fibers transduction via nonconcentrated LV (1 hour incubation with LV) muscle fibres did not express GFP, while it expressed desmin (red), however, branching out satellite cells expressed GFP. (c) Nontreated muscle fibers retain their cross-striation pattern and did not differ from muscle fibers transduced via nonconcentrated LV. Scale bar corresponds to 50 μm.

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