Primary Murine Myotubes as a Model for Investigating Muscular Dystrophy

Figure 3

Graph showing numbers of living muscle fibers depending on type of isolation. 1: trituration in nonconcentrated LV, incubation with LV–5 min, and plating direct on Geltrex-coated dish; 2: trituration in nonconcentrated LV, incubation with LV–3 h, and plating direct on Geltrex-coated dish; 3: trituration in nonconcentrated LV, incubation with LV–3 h, overnight preplating on non-coated dish, and then plating on Geltrex-coated dish; 4: trituration in DMEM supplemented with 20% FCS, incubation with concentrated LV–5 min, and plating direct on Geltrex-coated dish; 5: trituration in DMEM supplemented with 20% FCS, incubation with concentrated LV–3 h, and plating direct on Geltrex-coated dish ( dishes).