Primary Murine Myotubes as a Model for Investigating Muscular Dystrophy

Figure 6

Physiological properties of primary myotubes. (a) Primary myotubes responded to chemical and electrical stimulation within an increase in cytosolic Ca²⁺ followed by mitochondrial Ca²⁺ uptake. Cytosolic Ca²⁺ increase evoked by CmC or electrical stimulations and myotubes responded to stimulations by contraction and [Ca²⁺]_i increase, confirming developed system of DHPR and RyR. Scale bar corresponds to 50 μm. (b) Representative L-type Ca²⁺ current traces recorded in myotubes after seven days of differentiation (upper panel) and in muscle fibers (middle panel) in response to a series of 200 ms depolarizing steps from −30 to 40 mV in 10 mV increments (lower panel). (c) Current-voltage relationship in adult FDB muscle fibers and myotubes at seventh day of differentiation. Data are presented as mean ± SD ( cells).

(a)

(b)

(c)