Vitamin D Can Ameliorate Chlorhexidine Gluconate-Induced Peritoneal Fibrosis and Functional Deterioration through the Inhibition of Epithelial-to-Mesenchymal Transition of Mesothelial Cells
Effect of vitamin D on serum and aortic calcium and phosphate content. Sprague-Dawley rats received intraperitoneal (IP) injection of chlorhexidine gluconate (CG) daily with or without administration of low (L, 500 ng/kg), middle (M, 750 ng/kg), or high (H, 1 μg/kg) dose 1α,25(OH)2D3 (vit. D). Rats also received daily intraperitoneal instillation of normal saline (NS) as a control. Blood samples and aorta tissue samples were taken 29 days after the first IP injection. (a) 1α,25(OH)2D3 induced mild hypercalcemia in the low and middle dose groups but induced severe hypercalcemia (>15 mg/dL) in the high dose group. (b) However, no significant difference was found in the serum phosphate content of the different groups. (c and d) High dose vitamin D also induced severe aortic calcium and phosphate deposition. (e) Further, we analyzed serum 25(OH)D levels, and the results indicated that endogenous 25(OH)D was significantly suppressed after 1α,25(OH)2D3 treatment. Data are represented as mean ± S.D. (; ; ).