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BioMed Research International
Volume 2015, Article ID 597198, 12 pages
http://dx.doi.org/10.1155/2015/597198
Research Article

Biochemical Modulation of Lipid Pathway in Microalgae Dunaliella sp. for Biodiesel Production

1Microbial Biotechnology Department, Semnan University, Semnan 35131-19111, Iran
2Microbial Biotechnology and Biosafety Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj 31359-33151, Iran
3Biofuel Research Team (BRTeam), Karaj 31438-44693, Iran
4Genomics Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj 31359-33151, Iran
5Tropical Agro-Biomass (TAB) Group, Faculty of Plantation and Agrotechnology, Universiti Teknologi MARA (UiTM), 40450 Shah Alam, Malaysia
6Faculty of Engineering, Universiti Putra Malaysia (UPM), 43400 Serdang, Malaysia

Received 31 October 2014; Revised 7 January 2015; Accepted 8 January 2015

Academic Editor: Ximing Zhang

Copyright © 2015 Ahmad Farhad Talebi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Exploitation of renewable sources of energy such as algal biodiesel could turn energy supplies problem around. Studies on a locally isolated strain of Dunaliella sp. showed that the mean lipid content in cultures enriched by 200 mg L−1 myoinositol was raised by around 33% (1.5 times higher than the control). Similarly, higher lipid productivity values were achieved in cultures treated by 100 and 200 mg L−1 myoinositol. Fluorometry analyses (microplate fluorescence and flow cytometry) revealed increased oil accumulation in the Nile red-stained algal samples. Moreover, it was predicted that biodiesel produced from myoinositol-treated cells possessed improved oxidative stability, cetane number, and cloud point values. From the genomic point of view, real-time analyses revealed that myoinositol negatively influenced transcript abundance of AccD gene (one of the key genes involved in lipid production pathway) due to feedback inhibition and that its positive effect must have been exerted through other genes. The findings of the current research are not to interprete that myoinositol supplementation could answer all the challenges faced in microalgal biodiesel production but instead to show that “there is a there there” for biochemical modulation strategies, which we achieved, increased algal oil quantity and enhanced resultant biodiesel quality.