Diagnosis of Neisseria gonorrhoeae Using Molecular Beacon

Figure 1

Standardisation of use of moecular beacon for detection of N. gonorrhoeae. (a) Standardization of concentration of beacon. (b) Specificity of molecular beacons using unlabeled specific and nonspecific probe (1 : 0, 1 : 1, 1 : 5, 1 : 10, 1 : 20, and 1 : 50). (c) Sensitivity of molecular beacon using purified neisserial genomic DNA (100 fg to 10 ng). (d) Detection of clinical samples in ELISA reader. The amplified PCR products were transferred into wells of a 96-well plate containing 150 μL of 10 mM Tris/Cl (pH 8.0) and the fluorescence was measured using 492 nm excitation and 521 nm emission in ELISA reader. (e) Direct visualization of PCR tube under dark reader. Tube 1: NTC, tube 2: PTC, and tube 3: clinical sample (positive).

(a)

(b)

(c)

(d)

(e)