Lysophosphatidic Acid Inhibits Apoptosis Induced by Cisplatin in Cervical Cancer Cells

Figure 4

LPA effects on the expression of apoptotic proteins induced by cisplatin. Hela cells were treated with cisplatin (at 200 μg/mL) alone or plus LPA (at 20 μM) for 4 hours. Western blotting was used to determine the expression of apoptosis related proteins. β-actin was used as a protein loading control. (a) Western blotting of Fas-L. The increased Fas-L expression induced by cisplatin was significantly reversed by LPA treatment. (b) Western blotting of Bcl-2. LPA restored the Bcl-2 expression which was inhibited by cisplatin. (c) Western blotting of Bax. The upregulation of Bax expression induced by cisplatin was significantly inhibited by LPA.

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