Lysophosphatidic Acid Inhibits Apoptosis Induced by Cisplatin in Cervical Cancer Cells

Figure 6

Western blotting analysis of phosphorylated AKT in cells treated with LPA. (a) Hela cells were treated with LPA at different concentrations including 0, 0.1 μM, 1 μM, 5 μM, 20 μM, and 50 μM for 1 hour. Western blotting was conducted to detect the expression of phosphorylated AKT. Total AKT was detected and the results were used as protein input control. Densitometry analysis was carried out for quantification purpose. LPA treatment led to increased phosphorylation of AKT in a dose-dependent manner. (b) Time-response effect of LPA on the phosphorylation of AKT. Hela cells were treated with LPA at 20 μM for different times including 5 min, 10 min, 30 min, 1 hour, 2 hours, 3 hours, and 4 hours. Western blotting was used to detect the expression of phosphorylated AKT. Compared to the total AKT, LPA treatment activated the phosphorylation of AKT in a time-dependent manner.

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