Mammalian Cell Culture Process for Monoclonal Antibody Production: Nonlinear Modelling and Parameter Estimation

Table 3

Experimental concentration measurements [4, 27].

Time	0 h	28 h	54 h	76 h	101 h	124 h	147 h
GLC mM	3.59 ± 0.04	2.59 ± 0.09	1.88 ± 0.15	1.77 ± 0.05	1.70 ± 0.03	1.67 ± 0.04	1.68 ± 0.05
GLN mM	2.85 ± 0.04	1.27 ± 0.30	0.42 ± 0.31	0.11 ± 0.10	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00
ASN mM	0.46 ± 0.00	0.39 ± 0.02	0.35 ± 0.02	0.32 ± 0.03	0.28 ± 0.02	0.25 ± 0.02	0.22 ± 0.02
ASP mM	0.27 ± 0.02	0.18 ± 0.02	0.10 ± 0.04	0.07 ± 0.04	0.04 ± 0.04	0.03 ± 0.04	0.03 ± 0.04
LAC mM	0.51 ± 0.01	1.33 ± 0.06	1.66 ± 0.19	1.74 ± 0.01	1.71 ± 0.01	1.72 ± 0.01	1.73 ± 0.05
ALA mM	0.33 ± 0.03	0.72 ± 0.11	1.15 ± 0.17	1.32 ± 0.12	1.46 ± 0.07	1.48 ± 0.07	1.51 ± 0.08
PRO mM	0.30 ± 0.01	0.27 ± 0.02	0.42 ± 0.04	0.53 ± 0.02	0.56 ± 0.02	0.60 ± 0.01	0.60 ± 0.01
MAB 10^−4 mM	0.34 ± 0.12	1.02 ± 0.06	1.58 ± 0.16	2.31 ± 0.24	2.66 ± 0.41	3.09 ± 0.60	3.41 ± 0.75
BM mM	2.01 ± 0.20	11.61 ± 0.46	16.51 ± 0.85	17.98 ± 0.84	19.41 ± 2.21	18.67 ± 2.49	17.97 ± 1.33
[10^6 cells/mL	0.09 ± 0.01	0.58 ± 0.02	0.79 ± 0.05	0.72 ± 0.01	0.47 ± 0.06	0.17 ± 0.03	0.06 ± 0.02
[10^6 cells/mL	0.02 ± 0.01	0.05 ± 0.01	0.11 ± 0.01	0.25 ± 0.05	0.58 ± 0.05	0.85 ± 0.12	0.91 ± 0.07