Hepatocellular Carcinoma Growth Is Inhibited by Euphorbia helioscopia L. Extract in Nude Mice Xenografts

Figure 3

CyclinD1 protein expression in nude mouse xenografts. (a) Immunohistochemical staining detected CyclinD1 in xenografts. EAE treatment group hardly expressed CyclinD1 protein compared to control groups (red arrow). ×400 magnification. (b) Analysis of CyclinD1 protein expression in xenografts by Western blot. Total protein was extracted from xenografts and subjected to Western blot analyses. CyclinD1 protein expression was obviously reduced in EAE treatment groups compared with the control group (), especially in 200 μg/mL group (), different significantly. -Actin was used as the loading control.

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