Mushroom β-Glucan May Immunomodulate the Tumor-Associated Macrophages in the Lewis Lung Carcinoma

Figure 10

Effect of Antrodia camphorata beta-glucan on the amount of gene expressions of M-CSF, IL-6, IL-10, COX-2, and TGF-β in the tumor microenvironment of tumor-bearing mice. Tumor-bearing mice were fed with either twice-distilled water, celecoxib, Antrodia camphorata beta-glucan, or Ganoderma beta-glucan daily and euthanized after 12 days. Tumor tissues were then harvested (). RT-qPCR was used to quantify gene expressions of M-CSF, IL-6, IL-10, COX-2, and TGF-β. β-actin was used as the reference gene. (a) Amount of M-CSF gene expression. (b) Amount of IL-6 gene expression. (c) Amount of IL-10 gene expression. (d) Amount of COX-2 gene expression. (e) Amount of TGF-β gene expression. TC: fed with twice-distilled water; TM: fed with celecoxib; group TB: fed with Antrodia camphorata beta-glucan; group TX: fed with Ganoderma beta-glucan.

(a) M-CSF

(b) IL-6

(c) IL-10

(d) COX-2

(e) TGF-β