Mushroom β-Glucan May Immunomodulate the Tumor-Associated Macrophages in the Lewis Lung Carcinoma

Figure 11

Effect of Antrodia camphorata beta-glucan on the percentages of macrophages and proportions of M1 and M2 macrophages in the tumor microenvironment of tumor-bearing mice. Tumor-bearing mice were fed with distilled water, celecoxib, Antrodia camphorata beta-glucan, or Ganoderma beta-glucan daily and euthanized after 12 days. Tumor tissue samples were then harvested (). Flow cytometer was used to analyze percentages of macrophages and proportions of M1 and M2 macrophages in the tumor microenvironment. (a) Percentages of macrophages in the tumor. (b) Percentages of M1 macrophages in the tumor. (c) Percentages of M2 macrophages in the tumor. TC: fed with twice-distilled water; group TM: fed with celecoxib; group TB: fed with Antrodia camphorata beta-glucan; group TX: fed with Ganoderma beta-glucan.

(a) TAM

(b) M1

(c) M2