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BioMed Research International
Volume 2015, Article ID 607120, 13 pages
http://dx.doi.org/10.1155/2015/607120
Research Article

Endothelial Expression of Scavenger Receptor Class B, Type I Protects against Development of Atherosclerosis in Mice

1Lipoprotein Metabolism Section, Cardiovascular-Pulmonary Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
2Division of Diabetes, Endocrinology, and Metabolic Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
3Transgenic Core, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
4Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA

Received 30 April 2015; Revised 10 July 2015; Accepted 24 July 2015

Academic Editor: Pierre Sirois

Copyright © 2015 Boris L. Vaisman et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

In Supplementary Material we provide information about primers and probes used for genotyping Tie2-Scarb1 and LIV11-SCARB1 transgenic mice, for analysis of the transgenes condition (hetero- or homozygous) and for expression analysis by TaqMan assays of several individual genes, mentioned in the paper. In order to show the effect of EC specific expression of Scarb1 on atherosclerosis representative images of aortic lesions in the Tie2-Scarb1 transgenic mice on normal, ApoE-KO and Scarb1-KO backgrounds, as determined by en face analysis were included in Supplementary Fig. 1. In Supplementary Figure 2 it was shown that without expression of Scarb1 gene in liver additional expression of Tie2-Scarb1 was not able to change the level of plasma lipids.

  1. Supplementary Material