Screening and Identification of ssDNA Aptamer for Human GP73

Figure 5

Detection of GP73 in human liver tissues by the aptamer A10-2. Tissue-specific GP73 expression was studied in normal liver and HCC tissues by indirect immunohistochemistry, in which Dig-labeled A10-2 were used as molecular probe. In normal tissue, GP73 is expressed in biliary epithelial cells ((a) and (b)). In HCC tissue, the hepatocytes and biliary epithelial cells were both strong stained ((c) and (d)). Arrows indicate the location of GP73. (a) and (c): original magnification 200x. (b) and (d): original magnification 400x. The results were the representations of 20 normal and HCC tissue samples.

(a)

(b)

(c)

(d)