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BioMed Research International
Volume 2015, Article ID 612352, 5 pages
http://dx.doi.org/10.1155/2015/612352
Research Article

Sulfated Polysaccharides Isolated from Cloned Grateloupia filicina and Their Anticoagulant Activity

1Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao, Shandong 266071, China
2Qingdao Tumor Hospital, No. 127 Siliu Nan Road, Qingdao, Shandong 266042, China

Received 6 January 2015; Revised 17 March 2015; Accepted 17 March 2015

Academic Editor: Nuri Azbar

Copyright © 2015 Xiaolin Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Sulfated polysaccharides (GSP) were isolated from the cloned Grateloupia filicina which was cultured in Jiaozhou Bay, Qingdao, China. The yield of GSP was 15.75%. The total sugar and sulfate were 40.90 and 19.89%, respectively. And the average molecular weight was 11.7 KDa. The results of neutral sugar analysis showed that GSP was mainly sulfated polysaccharides of galactose. The experiments for activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) anticoagulant assays in vitro indicated that GSP was a good potential anticoagulant. Therefore, this study supplied new thought for the cloned Grateloupia filicina exploitation of high-value products.