Research Article

Molecular Detection and Sensitivity to Antibiotics and Bacteriocins of Pathogens Isolated from Bovine Mastitis in Family Dairy Herds of Central Mexico

Figure 2

Inhibitory detection of bacteriocins against bacteria using gel-screening assay. (a) SDS-PAGE; gel was overlaid with (b) Bacillus cereus 183 (control), (c) Raoultella sp., and (d) Staphylococcus agnetis. Bacteria (c) and (d) were isolated from bovines with mastitis. Lane 1, Morricin 269; lane 2, Kurstacin 287; lane 3, Kenyacin 404; lane 4, Entomocin 420; lane 5, Tolworthcin 524. Growth inhibition zones show the relative position of bacteriocins with molecular mass of ~10 kDa. Protein marker (BioRad) was used to estimate the molecular masses of bacteriocins.
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