The Macrophage Galactose-Type Lectin-1 (MGL1) Recognizes Taenia crassiceps Antigens, Triggers Intracellular Signaling, and Is Critical for Resistance to This Infection

Figure 3

MGL1^−/− macrophages display deficient tyrosine phosphorylation in response to TcSol. (a) MGL1^+/+ and MGL1^−/− bone marrow-derived Mϕ were synchronized in starvation conditions over night and then were stimulated or not with TcSol (50 μg/mL) for 5, 15 or 30 min. Next, total cell extracts were obtained and resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal antiphosphotyrosine antibody. Lane 1, serum-starved MGL1^+/+ Mϕs. Lanes 2–4, serum-starved MGL1^+/+ Mϕs following TcSol exposure for 5, 15 and 30 min, respectively. Lane 5, serum-starved MGL1^−/− Mϕs. Lanes 6–8, serum-starved MGL1^−/− Mϕs following TcSol exposure for 5, 15, and 30 min, respectively. Lanes 2–4 show an upregulation of the tyrosine phosphorylation of total proteins, which was sustained for at least 30 min (arrows). Lanes 6–8 show an unsustained tyrosine phosphorylation of proteins after similar TcSol stimulation of MGL1^−/− Mϕs. (b) Similar experiment using LPS (500 ng/mL) and/or TcSol stimulation for 30 min. (c) Densitometry of western blot placed in (b). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Western blots are representative of two independent experiments.

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