Silencing of Eag1 Gene Inhibits Osteosarcoma Proliferation and Migration by Targeting STAT3-VEGF Pathway

Figure 4

Eag1 knockdown suppresses adhesion, migration, and invasion of OS cells. (a) MG-63 and Saos-2 cells were transfected with indicated siRNAs and cell adhesion ability was evaluated by adhesion assay as described in Section 2 (, the results from scrambled siRNA group were given as 1.0). (b) MG-63 and Saos-2 cells transfection with scrambled siRNA or Eag1 siRNAs were wounded and then in fresh serum-free RPMI-1640 medium. After 24 h, migrating cells in the wound were counted (). (c) Representative pictures of the wounds at random locations. . (d) Representative microscopic images of the Transwell insert of transfected OS cells. MG-63 and Saos-2 cells were grown on Transwell inserts coated with collagen I and after transfection with scrambled siRNA or Eag1 siRNAs, invaded cells were counted. Each bar gives the mean ± SEM of 3 experiments. .

(a)

(b)

(c)

(d)