Organotypic Culture of Breast Tumor Explants as a Multicellular System for the Screening of Natural Compounds with Antineoplastic Potential

Figure 7

Effect of the combinations of paclitaxel with bioactive compounds on LDH release. Four tumor explants per treatment were incubated with combinations of TX and compounds for 48 h and then LDH activity was quantified by measuring the units of enzyme released into the supernatants from the culture medium. Control explants received no treatment. Concentrations in the combinations were 20 μg/mL + 100 μg/mL (TX + CA and TX + UA) and 20 μg/mL + 120 μg/mL (TX + RA). Results of LDH release are expressed as fold of change relative to control. Values represent means ± SD. Asterisks () indicate significant statistical differences ().

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