An Integrated Approach Using Spatial Analysis to Study the Risk Factors for Leishmaniasis in Area of Recent Transmission

Figure 4

Molecular detection and identification of Leishmania. (a) Nested PCR technique (LnPCR). Agarose gel (1.5%) of the second reaction of the LnPCR for the 353 bp fragment. Lanes: MW; 100 bp-ladder; 1 and 12, negative controls; 18, positive control; positive samples (6–9; 11; 14-15; and 17); negative samples (2–5; 10; 13; and 16). (b) Restriction fragment length polymorphisms (RFLP-PCR) of positive canine samples in the LnPCR. Agarose gel (4%) of PCR-RFPL fragments after enzymatic digestion with HaeIII. Lanes: MW, 100-bp ladder; 1, negative control; 2, L. amazonensis (IFLA/BR/67/PH8); 3, L. braziliensis (MHOM/BR/75/M2903); 4, L. infantum (MHOM/BR/74/PP75); 5 and 6, dogs infected with L. infantum, and 7, dog infected with L. braziliensis.

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