Loss of flfl Triggers JNK-Dependent Cell Death in Drosophila

Figure 3

flfl loss-of-function induced cell death was suppressed by compromised JNK activity. Light micrographs of Drosophila eyes ((a)–(d) and (f)–(h)) or acridine orange staining of eye discs from 3rd instar larvae ((a′)–(d′) and (f′)–(h′)) are shown. Compared with the control (a), knocking down flfl induced cell death in eye discs (b′) and a rough eye phenotype in adult (b), which were significantly suppressed by knocking down bsk ((c′) and (c)) or coexpressing a dominant negative form of Bsk ((d′) and (d)). Expressions of GFP-IR, bsk-IR, or were included as controls ((f)–(h′)). (e) is the statistical analysis of acridine orange positive cells in the posterior part of eye discs from the indicated panels. Column shows mean + SEM and significance was tested by unpaired Student -test;; .