Runx2/miR-3960/miR-2861 Positive Feedback Loop Is Responsible for Osteogenic Transdifferentiation of Vascular Smooth Muscle Cells

Figure 2

miR-2861 or miR-3960 promoted β-glycerophosphate-induced osteoblast differentiation of vascular smooth muscle cells. (a) Northern blot analysis of miR-2861 expression in VSMCs transfected with miR-C (control) or miR-2861 precursor (pre-miR-3861), miR-C, or pre-miR-3960. ((b) and (c)) ALP activity and osteocalcin secretion (b) and Western blot analysis of Runx2 protein expression (c) in 10 mM β-glycerophosphate- (β-GP-) treated VSMCs stably transfected with miR-C or pre-miR-2861, miR-C, or pre-miR-3960. Data are shown as mean ± s.e.m. .

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