Monitoring the Modifications of the Vitreous Humor Metabolite Profile after Death: An Animal Model

Table 1

Mean and standard deviation (SD) values of the spectral integrated area^a corresponding to the identified metabolites at each time point, with statistically significant Student’s t-test P values^b.

Metabolite	t0		t6			t12				t18				t24
	Mean	SD	Mean	SD	t0^c	Mean	SD	t6^d	t0^c	Mean	SD	t12^e	t0^c	Mean	SD	t1^f	t0^c
3-OH-But	0.7	0.1	0.71	0.06		0.7	0.1			0.5	0.1	**	*	0.5	0.06		*
Lac	20.6	0.8	26	2	**	30	2	*	***	28	3		**	24	2	**	***
Ala	0.4	0.1	0.42	0.02		0.49	0.03	**		0.54	0.02	**		0.54	0.03		*
GSH/GSSG	0.03	0.03	0.03	0.03		0.02	0.02		*	0.09	0.09			0.18	0.09	*	**
Cho	0.7	0.1	0.9	0.1	*	1.04	0.01			1.1	0.2		**	1.13	0.08		**
Gluc	0.8	0.3	0.52	0.05		0.27	0.07	***		0.09	0.02	**	*	0.078	0.008	*	*
Crt	0.7	0.2	0.6	0.1		0.66	0.05			0.9	0.2	*	*	0.9	0.1		*
MI	1.0	0.1	0.9	0.2		0.9	0.1			1.1	0.4			1.6	0.4	*	**
Hpx	0.05	0.02	0.111	0.004	**	0.137	0.004	***	***	0.16	0.03	*	***	0.17	0.01		***

Chemical shift range (ppm) of the corresponding integrated areas in the ¹H NMR spectra: Gluc 3.46–3.48, MI 4.06–4.08, Crt 3.92–3.94, Ala 1.46–1.48, 3-OH-But 1.20–1.22, Cho 3.20–3.22, Lac 1.34–1.36, GSH/GSSG 2.96–2.98, and Hpx 8.20–8.22; ^bP 0.01^***, ^**, ^*; ^cP values versus t0; ^dP values versus t6; ^eP values versus t12; ^fP values versus t18.