Application of AMOR in Craniofacial Rabbit Bone Bioengineering

Figure 3

Immunostaining reveals in situ BMP-2 and Osteocalcin protein expression. Rabbit calvaria surgical defect was treated with similar described treatments. Sections were labeled with anti-BMP-2 polyclonal Ab (a panel, brown), or anti-Osteocalcin polyclonal Ab (b panel, red) followed by HRP-conjugated secondary Ab. Immunohistochemistry revealed increased BMP-2 (a) and Osteocalcin (b) expression, in sites implanted with anti-BMP-2 mAb clones C22 and 3G7 at 6 weeks. Positive immunostaining of BMP-2 and Osteocalcin is indicated by black arrow. Negative staining for both molecules is represented by X symbol.

(a)

(b)