Effect of Laryngeal Squamous Cell Carcinoma Tissue Implantation on the Chick Embryo Chorioallantoic Membrane: Morphometric Measurements and Vascularity

Figure 1

Histological appearance of the control and experimental CAM and LSCC tissue. (a) Sight fields of CAM with LSCC implant; original magnification 4x. (b–d) The control CAMs: stained in H&Е (b); CAM’s blood vessels revealed with Sambucus nigra lectin (c, d). (e) Moderately differentiated squamous cell carcinoma of the larynx. Accumulation of atypical cells with concentrically arranged keratinized cells (“carcinoma pearls”: long arrows) was observed in the original LSCC tissue. (f) Thickened CAM and chorionic epithelium of experimental group with LSCC implant: invasion through epithelium (short arrows). (g) Increased vascularity under the LSCC implant. (b, c, e, f, and g) Bars 50 μm; original magnification 10x; (d) bar 20 μm; original magnification 40x.